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Identification of Reference Genes for Circadian Studies on Brain Microvessels and Choroid Plexus Samples Isolated from Rats.
Szczepkowska A, Harazin A, Barna L, Deli MA, Skipor J. Szczepkowska A, et al. Biomolecules. 2021 Aug 17;11(8):1227. doi: 10.3390/biom11081227. Biomolecules. 2021. PMID: 34439891 Free PMC article.
Delivery of putative compounds of therapeutic value to the brain is limited by brain barriers: the blood-brain barrier located in the endothelium of the brain microvessels (BrMVs) and the blood-cerebrospinal fluid barrier located in the epithelium of t …
Delivery of putative compounds of therapeutic value to the brain is limited by brain barriers: the blood-brain barrier …
Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio).
Rassier GT, Silveira TLR, Remião MH, Daneluz LO, Martins AWS, Dellagostin EN, Ortiz HG, Domingues WB, Komninou ER, Kütter MT, Marins LFF, Campos VF. Rassier GT, et al. Sci Rep. 2020 Jul 29;10(1):12692. doi: 10.1038/s41598-020-69423-y. Sci Rep. 2020. PMID: 32728128 Free PMC article.
The objective of the present study was to make a comprehensive analysis of stability of canditade RGs actb1, actb2, b2m, eif2s2, eef1a1, gapdh, rplp2, rpl7, rpl13alpha, tuba1, and rps18, in gh-transgenic and non-transgenic zebrafish. Liver, brain, intestine and musc …
The objective of the present study was to make a comprehensive analysis of stability of canditade RGs actb1, actb2, b2m, eif2s2, eef1a1, gap …
Progression-associated genes in astrocytoma identified by novel microarray gene expression data reanalysis.
MacDonald TJ, Pollack IF, Okada H, Bhattacharya S, Lyons-Weiler J. MacDonald TJ, et al. Methods Mol Biol. 2007;377:203-22. doi: 10.1007/978-1-59745-390-5_13. Methods Mol Biol. 2007. PMID: 17634619 Review.
We identified nine genes consistently dysregulated in high-grade tumors, using four novel tests for identifying differentially expressed genes. Four genes encoding ribosomal proteins (RPS2, RPS8, RPS18, RPL37A) were upregulated, and five genes (APOD, SORL1, SPOCK2, PRSS11, …
We identified nine genes consistently dysregulated in high-grade tumors, using four novel tests for identifying differentially expressed gen …
Systematic identification of human housekeeping genes possibly useful as references in gene expression studies.
Caracausi M, Piovesan A, Antonaros F, Strippoli P, Vitale L, Pelleri MC. Caracausi M, et al. Mol Med Rep. 2017 Sep;16(3):2397-2410. doi: 10.3892/mmr.2017.6944. Epub 2017 Jul 6. Mol Med Rep. 2017. PMID: 28713914 Free PMC article.
The present study conducted a meta-analysis of a pool of 646 expression profile data sets from 54 different human tissues and identified actin gamma 1 as the HK gene that best fits the combination of all the traditional criteria to be used as a reference gene for general use; two …
The present study conducted a meta-analysis of a pool of 646 expression profile data sets from 54 different human tissues and identified act …
A sequence-based approach to identify reference genes for gene expression analysis.
Chari R, Lonergan KM, Pikor LA, Coe BP, Zhu CQ, Chan TH, MacAulay CE, Tsao MS, Lam S, Ng RT, Lam WL. Chari R, et al. BMC Med Genomics. 2010 Aug 3;3:32. doi: 10.1186/1755-8794-3-32. BMC Med Genomics. 2010. PMID: 20682026 Free PMC article.
RESULTS: We show that (i) conventional references genes such as ACTB and GAPDH are highly variable between cancerous and non-cancerous samples, (ii) reference genes identified for lung cancer do not perform well for other cancer types (breast and brain), (iii) reference ge …
RESULTS: We show that (i) conventional references genes such as ACTB and GAPDH are highly variable between cancerous and non-cancerous sampl …
Identification of optimal reference genes for RT-qPCR in the rat hypothalamus and intestine for the study of obesity.
Li B, Matter EK, Hoppert HT, Grayson BE, Seeley RJ, Sandoval DA. Li B, et al. Int J Obes (Lond). 2014 Feb;38(2):192-7. doi: 10.1038/ijo.2013.86. Epub 2013 May 24. Int J Obes (Lond). 2014. PMID: 23736358 Free PMC article.
RESULTS: Using geNorm analysis, we found that it was sufficient to use the two most stably expressed genes as references in RT-qPCR analyses for each tissue under specific experimental conditions. B2M and RPLP0 in the hypothalamus, RPS18 and HMBS in the duodenum, RPLP2 and …
RESULTS: Using geNorm analysis, we found that it was sufficient to use the two most stably expressed genes as references in RT-qPCR analyses …
Analysis of Housekeeping Genes for Accurate Normalization of qPCR Data During Early Postnatal Brain Development.
Shaydurov VA, Kasianov A, Bolshakov AP. Shaydurov VA, et al. J Mol Neurosci. 2018 Mar;64(3):431-439. doi: 10.1007/s12031-018-1037-y. Epub 2018 Feb 28. J Mol Neurosci. 2018. PMID: 29492861
Using widely used GeNorm and NormFinder applications, as well as a novel approach, we compared stability of expression of GAPDH, YWHAZ, TFR1, RPS18, Rn18S, HPRT1, KIF5C, OSBP, and UQCRFS1. We found that, in both neocortical areas studied, YWHAZ and UQCRFS1 are the best ref …
Using widely used GeNorm and NormFinder applications, as well as a novel approach, we compared stability of expression of GAPDH, YWHAZ, TFR1 …
Genetic ablation of ataxin-2 increases several global translation factors in their transcript abundance but decreases translation rate.
Fittschen M, Lastres-Becker I, Halbach MV, Damrath E, Gispert S, Azizov M, Walter M, Müller S, Auburger G. Fittschen M, et al. Neurogenetics. 2015 Jul;16(3):181-92. doi: 10.1007/s10048-015-0441-5. Epub 2015 Feb 27. Neurogenetics. 2015. PMID: 25721894 Free PMC article.
Quantitative reverse transcriptase PCR in liver tissue validated >1.2-fold upregulations for the ribosomal biogenesis modulator Nop10, the ribosomal components Rps10, Rps18, Rpl14, Rpl18, Gnb2l1, the translation initiation factors Eif2s2, Eif3s6, Eif4b, Pabpc1 and the r …
Quantitative reverse transcriptase PCR in liver tissue validated >1.2-fold upregulations for the ribosomal biogenesis modulator Nop10, th …
Selection and validation of reference genes for qRT-PCR expression analysis of candidate genes involved in olfactory communication in the butterfly Bicyclus anynana.
Arun A, Baumlé V, Amelot G, Nieberding CM. Arun A, et al. PLoS One. 2015 Mar 20;10(3):e0120401. doi: 10.1371/journal.pone.0120401. eCollection 2015. PLoS One. 2015. PMID: 25793735 Free PMC article.
We monitored the expression profile of twelve reference genes: eEF-1alpha, FK506, UBQL40, RpS8, RpS18, HSP, GAPDH, VATPase, ACT3, TBP, eIF2 and G6PD. We tested the stability of their expression profiles in three different tissues (wings, brains, antennae), two devel …
We monitored the expression profile of twelve reference genes: eEF-1alpha, FK506, UBQL40, RpS8, RpS18, HSP, GAPDH, VATPase, ACT3, TBP …
Validation of endogenous reference genes in rat cerebral cortex for RT-qPCR analyses in developmental toxicity studies.
Ramhøj L, Axelstad M, Svingen T. Ramhøj L, et al. PeerJ. 2019 Jun 26;7:e7181. doi: 10.7717/peerj.7181. eCollection 2019. PeerJ. 2019. PMID: 31289700 Free PMC article.
The downstream target gene Pvalb was significantly downregulated in brains from young rats after in utero exposure to propylthiouracil (PTU), a medicinal drug inhibiting TH synthesis. ...For future studies examining relative gene expression in rat cerebral cortex under tox …
The downstream target gene Pvalb was significantly downregulated in brains from young rats after in utero exposure to propylthiouraci …